Fig. 9
From: Zoledronic acid blocks the interaction between breast cancer cells and regulatory T-cells
Chemokines expression in triple negative breast cancer cells treated with ZA. Relative mRNA levels of (a) CCL2 and (b) CCL5 were evaluated by qRT-PCR in MDA-MB-231 and MDA-MB-468 cells with or without 100 ng/ml IFN-γ activated in the presence of ZA (0, 5, 10 and 25 μM) for 24 h. Gene expression values were normalized to GAPDH expression. Concentrations of (c) CCL2 and (d) CCL5 were measured in the C.M. of 100 ng/ml IFN-γ treated MDA-MB-231 cells in the presence of ZA (0, 5, 10 and 25 μM) for 48 h by ELISA. Data were analyzed using one-way ANOVA; * P < 0.05, ** P < 0.01, *** P < 0.001