Fig. 8
From: Zoledronic acid blocks the interaction between breast cancer cells and regulatory T-cells
Influence of ZA on IDO mRNA expression by MDA-MB-231 and MDA-MB-468 cells. The mRNA levels of IDO were evaluated by qRT-PCR in 100 ng/ml IFN-γ  activated MDA-MB-231 and MDA-MB-468 cells treated with 0, 5, 10 and 25 μM ZA for 24 h. Gene expression values were normalized to GAPDH expression. Relative mRNA expression of IDO was calculated from cell treatment with ZA compared with cells only with IFN-γ stimulation. Values indicate means ± SEM of results from three independent experiments performed in duplicate (n = 3). * P < 0.05, *** P < 0.001