Fig. 6From: Zoledronic acid blocks the interaction between breast cancer cells and regulatory T-cellsInhibition effects of C.M. from ZA-pretreated MDA-MB-231 cells on Tregs immunosuppression function. a The percentage of responding effector T cells expressing activation marker CD69 in PBMC stimulated with anti-CD3/CD28 beads. b Tregs suppressed the percentage of responding effector T cells expressing activation marker CD69 in PBMC stimulated with anti-CD3/CD28 beads. c Tregs were cultured with C.M. of MDA-MB-231 cells and enhance the immunosuppression of Tregs to decrease responding effector T cells expressing activation marker CD69 in PBMC stimulated with anti-CD3/CD28 beads. d The percentage of immunosuppression was calculated using the following formulas: 100 – [(% CD69-positive in the presence of Tregs/% CD69-positive in the absence of Tregs) × 100]. Values shown are means ± SEM of results from three independent experiments. Significant differences were compared using one-way ANOVA; ** P < 0.01Back to article page