Fig. 6

Western analysis of p53 and SOD2. a Proteins from SUP-B15, CCRF-CEM and MOLT-4 cell lines treated with 10 μM daunorubicin for 4 h or untreated (control) were probed with anti-SOD2 or anti-phospho-p53 at Ser15. Separate gels from the same cell preparations were analysed for tubulin expression. b Evaluation of MnSOD2 expression. Tubulin expression was analysed as a loading control, results are expressed as ratio of SOD2 to tubulin intensity. Bars indicate mean of at least four replicates ± SEM, from two independent experiments. *** P < 0.001 one-way ANOVA