Fig. 4

Effect of daunorubicin on DSB. Measurement of DSB by γH2AX fluorescence for MOLT-4 (a), CCRF-CEM (b) and SUP-B15 (c) cells following 4 h treatment with 10 μM daunorubicin, followed by 4, 12 and 24 h (post recovery period) in recovery media. Median intensities from (flow cytometry histograms of the raw data) were used to plot the bar diagram. Graph indicates mean of total of six replicates ± SEM, from three independent experiments. Results were normalised to the control at each time point. * P < 0.05, ** P < 0.01; *** P < 0.001 one-way ANOVA followed by Tukey’s post-hoc test