Fig. 2

MUC16 CTD promotes, while the deletion of MUC16 inhibits, the proliferation and migration of EOC cells. SKOV3 cells were stably transfected with MUC16 CTD overexpression lentiviral vectors or empty vectors (EV). a Western blot is used to evaluate the expression of MUC16 CTD. b Real-time PCR is performed to analyze the RNA level of MUC16 CTD. Data are shown as the mean ± SEM (n = 3). ***p < 0.001 compared with the negative control (SKOV3 EV). c To knock out MUC16 in HO8910 cells, we designed two single guide RNA (sgRNA) targets to ensure the effect of MUC16 KO. d Knockout of MUC16 is also confirmed by western blot. e the downregulation of MUC16 in HO8910 cells is also confirmed by immunofluorescence. Data shown here are representative of 3 independent experiments. f, g The proliferation capacities of SKOV3 MUC16 CTD and HO8910 MUC16 KO are analyzed using MTS proliferation assay. Cell viability is determined by OD value. Data are shown as the mean ± SEM (n = 3). *p < 0.05 compared with SKOV3 EV. ***p < 0.001 compared with HO8910 WT. h, i A transwell assay is used to analyze the migration capacity of SKOV3 MUC16 CTD or HO8910 MUC16 KO cells; migrated cells are counted. Data are shown as the mean ± SEM (n = 3). *p < 0.05, ***p < 0.001. Data shown here are representative of 3 independent experiments