Fig. 4

cAMP Signalling signalling by CLR activated by its cognate agonists & receptor subtype can be discriminated using specific antagoninsts. Characterization of cAMP accumulation (30 min) in response to stimulation by αCGRP and AM in WK1 (a), JK2 (b), SB2b (c) and PB1 (d) cell lines. Data are analysed by three-parameter logistic curve. All values are mean + S.E.M. of 3 to 4 independent experiments conducted in triplicate. Receptor discrimination in cAMP accumulation (30 min) in response to stimulation by either αCGRP and AM alone, or in presence of the antagonists αCGRP(8–37) or AM(22–52) in WK1 (e), JK2 (f) and PB1 (g) cell lines. Data were fit using a three-parameter logistic curve. All values are mean + S.E.M. of 3 to 4 independent experiments conducted in triplicate