Skip to main content
Fig. 6 | BMC Cancer

Fig. 6

From: Hypoxia-induced cancer stemness acquisition is associated with CXCR4 activation by its aberrant promoter demethylation

Fig. 6

Activation of CXCR4 by its promoter DNA demethylation. a Real-time RT-PCR revealed significantly increased CXCR4 expression after treatment with either the DNA methyltransferase inhibitor (AZA) or hypoxic condition for 24 h compared with the controls in the lung cell lines. Data are presented as the mean ± SE (n = 4, *P < 0.05). b Methylation-specific real-time PCR revealed increased promoter demethylation in hypoxic cell groups compared with normoxic controls. Data are presented as mean ± SE (n = 4, *P < 0.05). c Bisulfite sequencing was performed to demonstrate the CpG site methylation status in the promoter region of CXCR4 in lung cell lines. Representative bisulfite sequencing results show 11 CpG sites presented in underlined letters within an 82-bp promoter region of CXCR4. CpG sites which were demethylated following hypoxic condition were demonstrated in red letters. Y indicates heterozygote C/T double peaks

Back to article page