Skip to main content
Fig. 3 | BMC Cancer

Fig. 3

From: Cancer-associated fibroblasts induce epithelial–mesenchymal transition of bladder cancer cells through paracrine IL-6 signalling

Fig. 3

iCAFs express IL-6 and induce the activation of the STAT3 signaling pathway in RT4 bladder cancer cells. a. Interleukin-6 (IL-6) mRNA expression levels in HFs, HFs + TGFß and iCAFs cells were determined by qPCR. b. The expression of the IL-6 protein was measured in supernatants from HFs, HFs + TGFß and iCAFs cells using enzyme-linked immunosorbent assay (ELISA). c. The presence of the IL-6 receptor (IL-6R) in iCAFs and RT4 cells was detected by Western blotting. d. The activation of the signal transducer and activator of transcription 3 (STAT3) and AKT signaling pathway in RT4 cells cultured in conditionned medium (CM) was evaluated by Western blotting. The graphs show mean +/− SD. The difference between groups was analyzed by one-way ANOVA followed by post hoc analysis using Dunnett’s multiple comparison tests. *P < 0.05, **P < 0.01, ***P = 0.001, n.s. = non-significant, n = 3–5

Back to article page