Different next-generation sequencing pipelines based detection of tumor DNA in cerebrospinal fluid of lung adenocarcinoma cancer patients with leptomeningeal metastases

Table 2 Feathers of the next-generation sequencing pipelines used for different sample types

	ddCAP	ddCAP on tissue	cHOPE	OncoAim
Number of Genes	10	10	59	59
Targets	all exons	all exons	hotspots	hotspots
Enrich method	targeted capture	targeted capture	multiple PCR	multiple PCR
DNA input	5~30 ng	50 ng	5~20 ng	10~20 ng
Instruments	NextSeq	NextSeq	NextSeq/miniSeq	NextSeq/miniSeq/MiSeq

ISSN: 1471-2407