Fig. 7
a- Summary of the mapping results for the reads by sample: KW-634, 821-LN, and 821-T4 untreated compared with treated cells. Each sample consists of reads that can be classified into the following categories: mapped (reads that align to the reference genome), outmapped or high abundance (e.g., rRNA, poly(A), poly(C), and mtRNA) and reads that do not align to anything (unmapped). b- The abundance of mtRNAs in cells treated with our Nano material compared with untreated cells in each cell line; there were no phenotypic effects or changes in the mitochondria but a significant reduction of cell growth. c- Endogenous BCL2 expression in mitochondria in mouse lung non-metastatic (KW-634, blue bars) and metastatic (821-T4, violet bars; 821-LN, green bars) cell lines, showing the various roles and effectiveness of miR-200c in each after treatment with 50 nM miR-200c and nano-miR-200c. The expression level of BCL2 was considerably elevated after treatment with miR-200c and Nano miR-200c in non-metastatic KW-634, reduced in metastatic 821-T4 and mixed in metastatic 821-LN. d- Similarly, Gtf2ird expression was slightly elevated in KW-634 (blue bars) treated with miR-200c/Nano miR-200c, decreased in 821-T4 (violet bars), and significantly elevated in 821-LN (orange bars)