Fig. 1

Macroscopic and histological effects of M30 on CTX-induced alopecia in C57BL/6 mice. The mice were depilated using wax strips and intraperitoneally injected with CTX with or without M30 supplement (see Methods section). After CTX treatment, shaved skin of the normal, CTX-treated, and M30-supplemented CTX-treated mice was photographically observed 2 weeks after CTX treatment. a Representative images of dorsal skin of normal mice (Normal), CTX-treated mice (CTX), and M30-supplemented CTX-treated mice (M30 + CTX) are shown. The sample tissue was sliced into 5-μm-thick transverse sections and these sections (n = 5) were observed using a confocal microscope. b Representative images of skin of normal mice (Normal), CTX-treated mice (CTX), and M30-supplemented CTX-treated mice (M30 + CTX) are shown. Scale bar = 100 μm