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Fig. 3 | BMC Cancer

Fig. 3

From: Droplet digital PCR as an alternative to FISH for MYCN amplification detection in human neuroblastoma FFPE samples

Fig. 3

Analysis of MYCN amplification by droplet digital PCR in human NB specimens. Representative one dimensional ddPCR plots for (a) MYCN and (b) reference gene RNAseP showing side-by-side comparison of MYCN copy number variations in MYCN amplified and non-amplified NB cases. MYCN was read in blue (FAM) channel, while RNASeP was read in green (HEX) channel. Each point represents a single droplet, which is scored as positive (colored and above the threshold intensity, as indicated by the pink line) or negative (grey, below the threshold line), depending on the fluorescent amplitude. (c) Representative (from amplified and non-amplified cases) two-dimensional scatter plots constructed with overlaid ddPCR data of the reference RNAseP (HEX) and MYCN (FAM) showing droplets containing no template (lower left, black), droplets containing only MYCN template (upper left, blue), droplets containing only reference RNAseP template (lower right, green), and droplets containing both MYCN and RNAseP templates (upper right, brown)

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