Fig. 4

suppression of miR-10b in cultured BT-549 cells increases cell proliferation and invasion. a mRNA levels of miR-10b target genes (relative to the appropriate scrambled controls) in cultured BT-549 cells transfected with oligonucleotides as indicated, qRTPCR. Shown are average values from experimental triplicates. Bars, stdev. *:p < 0.05 (t-test). b Quantification (using the CyQUANT® Direct Cell Proliferation Assay) of BT-549 cell proliferation 48 h after transfection with anti-miR-10b oligos, scrambled controls, and the TOX oligo as transfection control. Background-subtracted signals were normalized to the scrambled control – transfected cells. Shown are averages from 9 or more biological replicates. Bars: standard errors. *: p = 0.023 (t-test). c Quantification (using the CyQUANT® Direct Cell Proliferation Assay) of BT-549 cell invasion through Matrigel™-coated inserts, 48 h after transfection with anti-miR-10b and miR-10b- mimicking oligos or the appropriate scrambled controls. Background-subtracted signals were normalized to non-transfected cells. Shown are averages from 3 biological replicates. Bars: standard errors. *: p = 0.017 (t-test)