Fig. 4

CCNE1 amplification is associated with poor overall survival (n = 62, comprised of 55 patients from the “Discovery-TNBC” cohort and 7 patients from clinical report). (a) TNBCs with CCNE1 amplification (CN > 6) correlate with poor OS (p = 0.023); (b) No significant correlation was observed between patients’ RFS and CCNE1 amplification (p = 0.25), “CCNE1 amp = 0” represents tumors without CCNE1 amplification (CN < 6), “CCNE1 amp = 1” represents tumors with CCNE1 amplification (CN ≥ 6); (c) No significant correlation was observed between patients’ OS and CCNE1 mRNA expression level (p = 0.45), “CCNE1 mRNA change = 0” represents tumors without CCNE1 mRNA overexpression (below median), “CCNE1 mRNA change = 1” represents tumors with CCNE1 mRNA overexpression (above median); (d) Analysis of the association of CCNE1 gene expression and CCNE1 copy number (CN) alteration in primary and metastatic TNBCs showed that mean and variance of CCNE1 mRNA expression was comparable between primary and metastatic tumors. Mean CCNE1 gene expression in metastatic tumors was increased compared to primary tumors (expression = 5.29 vs. 3.87, SD = 0.49 vs. 2.02)