Fig. 7

Inhibition of macropinocytosis reduces vacuole formation and attenuates the activation of JNK in cells treated with MOMIPP. U251 cells were treated with MOMIPP, EIPA or a combination of both compounds for 4 h. a Cellular vacuolization was assessed by phase-contrast microscopy. b Immunoblot analysis was performed as described in the Methods. A representative blot is shown in the upper panel and the ratios of phosphorylated to total JNK (combined 54 kDa and 46 kDa splice variants) from three separate determinations are depicted in the bar graph