Fig. 1

MOMIPP selectively impairs glycolytic metabolism compared to the non-cytotoxic analog, MOPIPP. a One day after plating, U251 cells were treated with 10 μM MOMIPP or MOPIPP and examined by phase contrast microscopy after the indicated periods. b & c Five hours after addition of 10 μM MOMIPP or MOPIPP, U251 cells were subjected to metabolic flux analysis to assess oxidative function (OCR) or glycolytic function (ECAR), using a Seahorse® system, as described in the Methods. Three control and three drug-treated cultures were sampled at each time-point. Values are means (± SD), and significance of the differences between the readings at each point was assessed by Student’s unpaired t-test. In panel (c), the asterisks indicate that the ECAR values from the MOMIPP-treated cells were significantly different from the control values at all time points (p ≤ 0.05). The ECAR values for the MOPIPP-treated cells were not statistically different from the controls