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Fig. 8 | BMC Cancer

Fig. 8

From: CYR61 triggers osteosarcoma metastatic spreading via an IGF1Rβ-dependent EMT-like process

Fig. 8

CYR61 influences cell motility and the expression of EMT markers through a JNK-dependent pathway. a Migration of K7 M2 and U2OS cell lines incubated in the presence or absence of the JNK inhibitor SP600125 (15 µM), as assessed by wound healing assays. Pictures were taken at time 0 and 18 h after the wound. b Quantitative evaluation of the wound healing. Results are expressed as mean ± standard deviation. a: p < 0.05 vs. control cells; b: p < 0.05 vs. untreated. c Expression pattern of a panel of mesenchymal and epithelial markers in K7M2 cell lines cultured in the presence or absence of the JNK inhibitor SP600125, as assessed by RT-qPCR. The relative mRNA levels were calculated using the 2–ΔΔCT method and expressed as Log2 of fold change (mean ± standard deviation). a: p < 0.05 vs. control cells; b: p < 0.05 vs. untreated. d Expression pattern of a panel of mesenchymal and epithelial markers in K7M2 cell lines transfected with a MKK7-JNK expressing vector or the corresponding empty vector, as assessed by RT-qPCR. The relative mRNA levels were calculated using the 2–ΔΔCT method and expressed as Log2 of fold change (mean ± standard deviation). a: p < 0.05 vs. control cells; b: p < 0.05 vs. untreated

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