Fig. 7
From: CYR61 triggers osteosarcoma metastatic spreading via an IGF1Rβ-dependent EMT-like process
CYR61 influences cell motility and cell-cell interaction through the IGF1/IGF1Rβ pathway. a Migration of K7M2 and U2OS cell lines incubated in the presence or absence of recombinant IGF1 or neutralizing anti-IGF1 antibody, as assessed by wound healing assays. Pictures were taken at time 0 and 18 hrs after the wound. b Quantitative evaluation of the wound healing. Results are expressed as mean ± standard deviation. a: p < 0.05 vs. control cells. c Cell aggregation assay of K7M2 and U2OS cell lines cultured in the presence or absence of neutralizing anti-IGF1 antibody. d Quantitative evaluation of the relative aggregate surface. Results are expressed as mean ± standard deviation. a: p < 0.05 vs. control cells; b: p < 0.05 vs. untreated. e Quantitative evaluation of the relative number of particles. Results are expressed as mean ± standard deviation. a: p < 0.05 vs. control cells; b: p < 0.05 vs. untreated