Fig. 4
From: CYR61 triggers osteosarcoma metastatic spreading via an IGF1Rβ-dependent EMT-like process
CYR61 controls osteosarcoma cell phenotype in vitro. a Expression pattern of a panel of mesenchymal and epithelial markers in K7 M2 and U2OS cell lines, as assessed by RT-qPCR. The relative mRNA levels were calculated using the 2–ΔΔCT method and expressed as Log2 of fold change (mean ± standard deviation). a: p < 0.05 vs. control cells. b Expression pattern of CYR61, N- and E-cadherin proteins in K7 M2 and U2OS cell lines, as assessed by Western blot. Actin was used as loading control. c Spearman correlation between N−/E-cadherin ratio and CYR61 expression level in K7 M2 and U2OS cell lines. The black line shows the regression line