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Fig. 1 | BMC Cancer

Fig. 1

From: Ago HITS-CLIP expands microRNA-mRNA interactions in nucleus and cytoplasm of gastric cancer cells

Fig. 1

Acquirement of Ago2 complexes from cytoplasm and nucleus of gastric cancer cells. a Isolation of nuclei and cytoplasm. The nuclei and cytoplasm of gastric cancer cells (MGC-803) were isolated and then examined by Western blot analysis. Antibody against β-tubulin, a marker of cytoplasm, or Lamin A/C, a marker of nucleus was indicated on the right. b Co-immunoprecipitation of Ago2 complex of isolated nucleus and cytoplasm. Co-IP was conducted using isolated nucleus or cytoplasm with Ago2-specific antibody. The Co-IP products were detected by Western blot to detect the Ago2 protein

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