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Table 2 Primary antibodies used by Western blotting and immunofluorescence studies

From: Podoplanin (PDPN) affects the invasiveness of thyroid carcinoma cells by inducing ezrin, radixin and moesin (E/R/M) phosphorylation in association with matrix metalloproteinases

Targeted protein

Other characteristics

Antibody name (manufacturer)

podoplanin

mouse mAb

D2–40 (Biorad, AbD Serotec, USA)

phospho-ERM

rabbit mAb

48G2 (Cell Signaling, USA)

phospho-p44/42 MAPK (Erk1/2)

rabbit mAb

20G11 (Cell Signaling, USA)

FAK

rabbit Ab

Phospho FAK (Tyr397) (Cell Signaling, USA)

CD44

mouse mAb

156-3C11 (Cell Signaling, USA)

paxillin

rabbit Ab

H-114 (Santa Cruz, USA)

vimentin

mouse mAb

Vim 3B4 (Abcam, UK)

snail

goat Ab

ab53519, (Abcam, UK)

twist

rabbit Ab

ab50581, (Abcam, UK)

RhoA

rabbit mAb

67B9 (Cell Signaling, USA)

  1. mAb: monoclonal antibody