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Table 1 Sequences of used qPCR primers

From: Podoplanin (PDPN) affects the invasiveness of thyroid carcinoma cells by inducing ezrin, radixin and moesin (E/R/M) phosphorylation in association with matrix metalloproteinases

Gene name Forward primer sequence Reverse primer sequence
PDPN 5’-CGAAGATGATGTGGTGACTC-3′ 5’-CGATGCGAATGCCTGTTAC-3′
EZR 5′- TCTTCGCTGCTGCTGGATAG-3′ 5’-GGTGGTAACTCGGACATTGATTG-3′
MSN 5’-TGAGGCTGTGGAGTGGCAGC-3′ 5’-CTAGAGGCTGGGTGCCCATT-3′
RDX 5’-GGCAACACAAAGCTTTTGCA-3′ 5’-ATATATGCAAAATAACAGCTC-3′
MMP1 5’-CTGGCCACAACTGCCAAATG-3′ 5’-CTGTCCCTGAACAGCCCAGTACTTA-3′
MMP2 5’-CAGGGAATGAGTACTGGGTCTATT-3′ 5’-ACTCCAGTTAAAGGCAGCATCTAC-3′
MMP3 5’-GAAATGAGGTACGAGCTGGATACC-3’ 5’-ATGGCTGCATCGATTTTCCT-3’
MMP7 5’-GCCTACCTATAACTGGAATG-3’ 5’-AGCCTTTGACACTAATCG-3’
MMP9 5’-GCACGACGTCTTCCAGTACC-3’ 5’-CAGGATGTCATAGGTCACGTAGC-3’
MMP13 5’-TCCCAGGAATTGGTGATAAAGTAGA-3’ 5’CTGGCATGACGCGAACAATA-3’
SNAI1 5’-CCCAATCGGAAGCCTAACTA-3’ 5’-CAGGACAGAGTCCCAGATGAG-3’
TWIST1 5’-CATCGACTTCCTCTACCAGGTC-3’ 5’-TCCATTTTCTCCTTCTCTGGAA-3’
RHOA 5’-TGCTTGCTCATAGTCTTCAG-3’ 5’-CACATCAGTATAACATCGGTATC-3’
PTK2 5’-GGTGCAATGGAGCGAGTATT-3’ 5’-GCCAGTGAACCTCCTCTGA-3’
PXN 5’-ACGTCTACAGCTTCCCCAACAA-3’ 5’-AGCAGGCGGTCGAGTTCA-3’
VIM 5’-GACAATGCGTCTCTGGCACGTCTT-3’ 5-TCCTCCGCCTCCTGCAGGTTCTT-3’
18S rRNA 5’-CCAGTAAGTGCGGGGTCATAAG-3’ 5’-CCATCCAATCGGTAGTAGCG-3’