Table 1 Sequences of used qPCR primers
Gene name | Forward primer sequence | Reverse primer sequence |
|---|---|---|
PDPN | 5’-CGAAGATGATGTGGTGACTC-3′ | 5’-CGATGCGAATGCCTGTTAC-3′ |
EZR | 5′- TCTTCGCTGCTGCTGGATAG-3′ | 5’-GGTGGTAACTCGGACATTGATTG-3′ |
MSN | 5’-TGAGGCTGTGGAGTGGCAGC-3′ | 5’-CTAGAGGCTGGGTGCCCATT-3′ |
RDX | 5’-GGCAACACAAAGCTTTTGCA-3′ | 5’-ATATATGCAAAATAACAGCTC-3′ |
MMP1 | 5’-CTGGCCACAACTGCCAAATG-3′ | 5’-CTGTCCCTGAACAGCCCAGTACTTA-3′ |
MMP2 | 5’-CAGGGAATGAGTACTGGGTCTATT-3′ | 5’-ACTCCAGTTAAAGGCAGCATCTAC-3′ |
MMP3 | 5’-GAAATGAGGTACGAGCTGGATACC-3’ | 5’-ATGGCTGCATCGATTTTCCT-3’ |
MMP7 | 5’-GCCTACCTATAACTGGAATG-3’ | 5’-AGCCTTTGACACTAATCG-3’ |
MMP9 | 5’-GCACGACGTCTTCCAGTACC-3’ | 5’-CAGGATGTCATAGGTCACGTAGC-3’ |
MMP13 | 5’-TCCCAGGAATTGGTGATAAAGTAGA-3’ | 5’CTGGCATGACGCGAACAATA-3’ |
SNAI1 | 5’-CCCAATCGGAAGCCTAACTA-3’ | 5’-CAGGACAGAGTCCCAGATGAG-3’ |
TWIST1 | 5’-CATCGACTTCCTCTACCAGGTC-3’ | 5’-TCCATTTTCTCCTTCTCTGGAA-3’ |
RHOA | 5’-TGCTTGCTCATAGTCTTCAG-3’ | 5’-CACATCAGTATAACATCGGTATC-3’ |
PTK2 | 5’-GGTGCAATGGAGCGAGTATT-3’ | 5’-GCCAGTGAACCTCCTCTGA-3’ |
PXN | 5’-ACGTCTACAGCTTCCCCAACAA-3’ | 5’-AGCAGGCGGTCGAGTTCA-3’ |
VIM | 5’-GACAATGCGTCTCTGGCACGTCTT-3’ | 5-TCCTCCGCCTCCTGCAGGTTCTT-3’ |
18S rRNA | 5’-CCAGTAAGTGCGGGGTCATAAG-3’ | 5’-CCATCCAATCGGTAGTAGCG-3’ |