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Fig. 8 | BMC Cancer

Fig. 8

From: Podoplanin (PDPN) affects the invasiveness of thyroid carcinoma cells by inducing ezrin, radixin and moesin (E/R/M) phosphorylation in association with matrix metalloproteinases

Fig. 8

Effect of MAPK pathway inhibition on PDPN expression in BcPAP and TPC1 cell lines. a. RT-qPCR analysis of PDPN mRNA levels in BcPAP and TPC1 cells 24 h after treatment with 10 μM PLX4720 (BRAF V600E inhibitor), 50 μM U0126 (MEK1/2 inhibitor), or DMSO as a negative control. The results were normalized to 18S rRNA levels. The bars represent the average fold change in transcript quantity in cells treated with the inhibitors compared to cells treated with DMSO. The results are representative of three independent experiments. Data are presented as the means ± standard errors of the mean (SEM) * < P,0.05; ** < P,0.01. b. Western blot analysis of PDPN, phosphorylated ERK and β-actin protein levels in TPC1 and BcPAP cells 24 h after treatment with 10 μM PLX4720, 50 μM U0126, or DMSO as negative control

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