Fig. 5

Silencing of PDPN changes cytoskeleton organization in TPC1 and BcPAP cells. Immunofluorescent staining of cytoskeleton in TPC1 and BcPAP cells transfected with siPDPN or control siNeg. To visualize filamentous actin (F-actin), cells were fixed with with 4% PFA/PBS, permeabilized with 0.1% Triton X-100, and stained with Phalloidin-FITC (green), and counterstained with DAPI (blue). Magnification: 630x