Fig. 2

Cell cycle shift by CKI and changing expression of key proteins. a Histogram and statistical results of cell cycle shift regulated by CKI over 48 h. In both cell lines, the earliest shifted cell cycle phase was S phase 6 h after treatment. Compared to Hep G2, MDA-MB-231 showed delayed responses. b Expression levels for five proteins as a result of CKI treatment at both 24 and 48 h. Statistical analyses were performed using two-way ANOVA comparing treated with untreated (*p <0.05, **p <0.01, ***p <0.001, ****p <0.0001); bars show one standard deviation from the mean