Table 1 The sequence of primers and probes used for genes expression
Targeted gene | Sequence 5’→3’ | Purpose | Product length (bp) |
|---|---|---|---|
AKT1 | 5’-GTGTCAGCCCTGGACTACC-3′ 5’-CAGCCCGAAGTCTGTGATCTTA-3’ FAM-TCCTTGTCCAGCATGAGGTTCTCCAGC-BHQ | Forward Reverse Probe | 114 |
BAD | 5’-GACGAGTTTGTGGACTCCTTTAAG-3′ 5’-CCTGCCCAAGTTCCGATCC-3’ FAM-TCCTCGCCCGAAGAGCGCGG-BHQ | Forward Reverse Probe | 129 |
RORγt | 5’-GCTAGGTGCAGAGCTTCAGG-3′ 5’-TGTTCTCATGACTGAGCCTTGG-3’ FAM-CCTTGGCTCCCTGTCCTTCTCAGCA-BHQ | Forward Reverse Probe | 145 |
FOXP3 | 5′- ACTACTTCAAGTTCCACAACATGC -3′ 5′- GAGTGTCCGCTGCTTCTCTG-3’ FAM- TCACCTACGCCACGTTCATCCGCT -BHQ | Forward Reverse Probe | 95 |
IFNλ3 | 5’-GTGGCTTTGGAGGCTGAG-3′ 5’-GCTGGTCCAAGACATCCC-3’ FAM-TTCTGGAGGCCACCGCTGAC-BHQ | Forward Reverse Probe | 91 |
HBZ | 5’-AGAACGCGACTCAACCGG-3′ 5’-TGACACAGGCAAGCATCGA-3’ FAM-TGGATGGCGGCCTCAGGGCT-BHQ | Forward Reverse Probe | 133 |
β2M | 5’-TTGTCTTTCAGCAAGGACTGG-3′ 5’-CCACTTAACTATCTTGGGCTGTG-3’ FAM-TCACATGGTTCACACGGCAGGCAT-BHQ | Forward Reverse Probe | 127 |