Fig. 6

Loss of SEMA5A activates the PI3K/AKT pathway. a-b Western blots of whole cell lysates showing an increase in levels of p-PI3K (Ser 458), p-AKT (Ser 473) and GSK3β (Ser9) in T3M-4-shSEMA5A (a) in comparison with T3M-4-Control cells and CD18/HPAF-shSEMA5Acells (b) in comparison with CD18/HPAF-Control cells suggesting activation of PI3K/AKT signaling. GAPDH serves as a loading control. Western blots show no change in the total protein levels of PI3K, AKT, and GSK3β. The intensity of the bands in Western blot analysis was quantified by Image J using GAPDH as an internal control and was normalized with respect to the T3M-4-Control cells or CD18/HPAF-Control cells