Fig. 5

Overexpression of shRNA-resistant Sema5A rescues epithelial phenotype. a-b Western blot analysis is showing an increase in SEMA5A and decrease in β-catenin expression in T3M-4-shSEMA5A (a) and CD18/HPAF-shSEMA5A cells (b) transfected with control pBK-CMV vector and full-length mouse Sema5A. GAPDH was used as loading control. c Increase in E-Cad and decrease in N-cad protein expression with respect to β-actin as a loading control in T3M-4-shSEMA5A cells transfected control pBK-CMV vector and full-length mouse Sema5A. d Increase in E-Cad and decrease in Snail protein expression with respect to GAPDH as a loading control in CD18/HPAF-shSEMA5A cells transfected control pBK-CMV vector and full-length mouse Sema5A. The intensity of the bands in Western blot analysis was quantified using Image J software and was normalized with respect to the T3M-4-shSEMA5A or CD18/HPAF-shSEMA5A cells transfected with control pBK-CMV vector