Fig. 3

TIMP-2 activity and ECM adhesion. a TIMP-2 activity in melanoma cells following treatment with CaG or IQG N-glycan for two days. b TIMP-2 activity of CaG or IQG N-glycan in HUVEC cells in pretreated with TNF-alpha. Each value represents mean ± SD. N = 3, *p < 0.05 vs CON (PBS) group. TIMP-2, a secreted protein known to prevent degradation of the ECM, was increased by N-glycan from CaG in both melanoma and HMVEC endothelial cells. Otherwise, TIMP-2 level of N-glycan from IQG was selectively decreased in melanoma cells so that ECM of melanoma could be degraded. c ECM adhesion activity (microarray) colorimetric detection of N-glycan of CaG or IQG on melanoma cells incubated with various extracellular matrix adhesion molecules: collagen, fibronectin, laminin, tenascin and vibronectin. Both N-glycans from CaG and IQG were found to have high ECM adehesion activity for Collagen I. Each value represents mean ± SD. N = 3