Fig. 6
Ras-ERK1/2 regulated H3K9ac via MDM2-mediated PCAF degradation. SW48 cells were transfected with pEGFP-K-RasG12V/T35S, PCAF-HA, MDM2-His (with increasing amount 0.5, 1, and 2 g) and pEGFP-N1. a Exogenous and b endogenous expression of PCAF was measured by Western blot. SW48 cells were transfected either by MDM2-His (2 g) or by MDM2 mutated type (MDM2-MU), then c exogenous and d endogenous expression of PCAF were reassessed. Anti-His and anti-HA antibodies were used for testing the exogenous levels of MDM2 and PCAF, respectively. e MDM2 and H3K9ac expression in cells transfected with pEGFP-K-RasG12V/T35S or pEGFP-N1. f The protein level of MDM2 after siRNA transfection was tested. g After the indicated transfection, H3K9ac level was tested by Western blot