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Fig. 5 | BMC Cancer

Fig. 5

From: RETRACTED ARTICLE: Ras-ERK1/2 signaling contributes to the development of colorectal cancer via regulating H3K9ac

Fig. 5

Ras-ERK1/2 repressed H3K9 acetylation in SW48 cells via degradation of PCAF. a The mRNA level of PCAF after the indicated transfected was tested. b and c Western blot analysis was performed to measure the expression of PCAF, HDAC2, and H3K9ac following the indicated transfection. Anti-HA antibody was used for testing the exogenous levels of PCAF and HDAC2. d ChIP analysis for testing PCAF levels when different genes were expressed. e 25 μM of MG132 was used to treat cells, after which Western blot was performed to reassess PCAF level. Protein expression of H3K9ac was monitored in the f absence or g presence of MG132. ** P < 0.01; *** P < 0.001

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BMC Cancer

ISSN: 1471-2407

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