Fig. 1
Ras-ERK1/2 repressed H3K9 acetylation in SW48 cells. SW48 cells were transfected with empty pEGFP vector, pEGFP-K-Ras-WT (wild type) or pEGFP-K-RasG12V/T35S construct. Protein levels of a p-ERK1/2 and b and c H3K9ac were measured by Western blot analysis. ** P < 0.01. Four inhibitors specific for ERK1/2, MAPK, PI3K and JNK pathways, i.e., SCH772984, SB203580, LY294002 and SP600125 were used to treat cells. Protein levels of d H3K9ac and e p-ERK1/2 were measured by Western blot analysis