Skip to main content

Table 1 Summary of compound-induced cross-linking of linear pUC19 DNA

From: The interactions of novel mononuclear platinum-based complexes with DNA

Compounda

ICLE (%)

Drug concentration at 50% dsDNA retention (μM)

rb (drug: nucleotide) at 50% dsDNA retention

Last detected data point (μM)

Cisplatin

6.8 ± 0.5

0.09 ± 0.01

1.9 × 10− 3 ± 1.4 × 10− 4

10

[Pt(RR-DACH)Cl2]

5.6 ± 2.1

0.13 ± 0.05

2.7 × 10− 3 ± 9.9 × 10− 4

3

[Pt(RR-DACP)Cl2]

3.1 ± 0.3

0.21 ± 0.02

4.1 × 10− 3 ± 3.9 × 10− 4

30

[Pt(SS-DACP)Cl2]

5.1 ± 0.2

0.12 ± 0.01

2.5 × 10− 3 ± 1.1 × 10− 4

3

[Pt(4FH)Cl2]

0.04 ± 0.02

23.52 ± 8.94

4.7 × 10− 1 ± 1.8 × 10− 1

300

  1. aThe rb value was calculated based on 2 × 10− 9 mol of nucleotide sample incubated with each compound. The ‘last detected data point’ refers to the highest drug concentration at which the DNA was still detectable on the agarose gel. All values and their respective SEM values were determined from three separate experiment
Back to article page