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Fig. 3 | BMC Cancer

Fig. 3

From: The interactions of novel mononuclear platinum-based complexes with DNA

Fig. 3

Electropherogram traces showing DNA damage induced by cisplatin and novel platinum-based compounds that was amplified by the LAR. a The G dideoxy sequencing lane used to determining the location of G-bases in the PvuII-cleaved pUC19/T7 sequence. b The 5% (v/v) DMF treated DNA, a negative control. DNA damage induced by (c) 0.3 μM cisplatin, (d) 0.3 μM [Pt(RR-DACP)Cl2] (e) 0.3 μM [Pt(SS-DACP)Cl2] and (f) 30 μM [Pt(4FH)Cl2]. The relative fluorescence intensity is plotted on the y-axis (left) and the DNA fragment size (bp) is plotted on the x-axis (top). The primer annealing site and full length extension product of 186 bp are highlighted. Damage peaks corresponding to sites of telomeric repeats T1 to T7 and the G-rich sites (G3I, G3II, G4 and G5) are labelled accordingly

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