Fig. 4

BRK protein and transcript levels are elevated in ER-positive breast cancer cells. a Lysates from 184B5, a transformed cell line established from normal mammary tissue, and 17 breast cancer cell lines, with cell lines subtype as defined by Neve and colleagues Neve et al. [22], were evaluated for BRK and ERα expression by immunoblotting. β-tubulin was used as the loading control. b and c BRK and ERα mRNA expression was assessed by quantitative reverse transcriptase PCR (qPCR). NT = normal transformed, E = ER-positive, T = triple negative, H=HER2, N = normal