Fig. 6
From: GRP78 modulates cell adhesion markers in prostate Cancer and multiple myeloma cell lines
Functional changes in PC3 adherence to OSB after GRP78 KD or incubation with a N-cad NAb clone CG-4 (N-cad NAb). PC3 cells were fluorescently labeled and re-seeded into 96-well plates with confluent OSB 48 h post-transfection. Percentage of adhered cells was determined 24 h after co-culture. a Representative image of PC3 + OSB co-culture. Left = brightfield, right = composite. b Fluorescent images of labeled PC3 cells in supernatant after being removed from the co-culture and are representative of 3 independent trials. *P < 0.05 and ***P < 0.001 compared to control (untreated) cells for the N-cad NAb or the GRP78 siRNA, respectively. Scale bar = 50 μm