Fig. 3
From: GRP78 modulates cell adhesion markers in prostate Cancer and multiple myeloma cell lines
Effects of GRP78 silencing on GRP94 and GRP75 in PC3 cells. 50 nM siRNA cocktail against GRP78 or control siRNA were transfected into PC3 cells. Total mRNA and protein levels were analyzed at 48 h and 72 h, respectively. a Western blot of GRP protein expression after GRP78 silencing. b Protein expression analysis. Target protein levels were normalized against the loading control GAPDH and compared to control siRNA. Blot bands and quantitative values are presented as the mean ± SD and representative of 3 separate trials. c qRT-PCR analysis of relative mRNA levels of GRPs upon GRP78 silencing. Target mRNA levels are relative to the control siRNA and represented as the mean fold change ± SD of 3 separate trials. ***P < 0.001 for GRP94. d Viability assay PC3 cells after siRNA transfection. 50 nM siRNA cocktail or a control siRNA were transfected into PC3 cells and analyzed by flow cytometry at 48 and 72 h. Histograms are representative of 3 independent trials at each time point