Skip to main content
Fig. 2 | BMC Cancer

Fig. 2

From: GRP78 modulates cell adhesion markers in prostate Cancer and multiple myeloma cell lines

Fig. 2

Effect of ER stress on GRP78 and N-cad mRNA levels in MM.1S cells. a Effect of ER stress inducers on MM.1S cells. Cells were treated with 10 nM bortezomib (BTZ) or 1 nM thapsigargin (Tg). qRT-PCR analysis was performed after 18 h of consecutive treatment. **P < 0.01 and ****P < 0.001 for GRP78 with BTZ treatment and Tg treatment vs. vehicle control (DMSO), respectively. ****P < 0.0001 for N-cad with both BTZ and Tg treatment vs. vehicle control (DMSO). b GRP78 silencing after BTZ treatment. MM.1S cells were treated overnight with 10 nM BTZ followed by treatment with 100 nM siRNA cocktail against GRP78. qRT-PCR analysis was performed 48 h post-transfection. ***P < 0.001 and P < 0.05 for GRP78 and N-cad vs. BTZ only treatment, respectively. c Effect of hypoxia on MM.1S cells. Cells were exposed to either 3% O2 for 72 h or 1% O2 for 24 h. Relative mRNA levels were compared to cells exposed to normoxia. ***P < 0.001 and **P < 0.01 for GRP78 (3% O2 and 1% O2). ****P < 0.0001 and **P < 0.01 for N-cad (3% O2 and 1% O2). All target mRNA levels are represented as the mean fold change ± SD of 3 separate trials

Back to article page