Fig. 1

Propofol suppressed A549 cell growth, but induced cell apoptosis. After 2–10 μg/mL propofol treatment, (a and b) the viability of A549 and BEAS-2B cells was detected using CCK-8 assay. After 8 μg/mL propofol treatment, (c) the proliferation of A549 cells was measured using BrdU incorporation assay, (d) the protein expressions of p53, p16 and Cyclin D1 in A549 cells was assessed using western blotting, (e) the apoptosis of A549 cells was determined using Annexin V-FITC/PI staining and flow cytometry, and (f) the protein expressions of Bcl-2, Bax, cleaved-Caspase 3 and cleaved-Caspase 9 in A549 cells were assessed using western blotting. N = 3.*P < 0.05, **P < 0.01, ***P < 0.001