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Fig. 3 | BMC Cancer

Fig. 3

From: A novel detection methodology for HER2 protein quantitation in formalin-fixed, paraffin embedded clinical samples using fluorescent nanoparticles: an analytical and clinical validation study

Fig. 3

a HER2 expression was measured using FACS and PID in six types of cell lines. The test was conducted as 5 independent tests. Results of FACS and PID (per cell) were analyzed using Pearson correlation coefficient. Pearson correlation coefficient was 0.99. b HER2 expression was measured using PID in six types of cell lines. The test was conducted as 5 independent tests. Each experiment consisted of 5 slides. Blank experiment consisted of 3 slides (Blank = without primary antibody). A total of 25 slides were stained in each cell lines and 15 slides were stained in blank test. The separation between the each cell lines were tested by multiple comparison using Kruskal-Wallis test and Student’s t-test in each other. PID score of all 6 cell lines were statistical significantly different. c Analytical sensitivity test using HER2-expressing cell lines. PID score compared with blank test that was stained without primary antibody. In MCF7 cell line, the PID score / cells was 5.8 times that of the blank test and in T47D cell line, the PID score / cells was 15.0 times that of the blank test. d Schematic for reproducibility and robustness test on a single cell line. Twelve cut sections from one FFPE cell block were cut on 5 different days. Nine slides were tested with PID/IHC; three slides were tested without primary antibody (Blank) by four different technicians. A coefficient of variation of 6.7% for all the 5 days was obtained. Within-run variation ranged from 3.6 to 10.7%

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