Fig. 3

The transmigration stimulated by LPA 18:1 and profiling of breast (cancer) cell lines: (a) The ratio of transmigrated cells compared to the control (y-axis) in varying LPA concentrations (x-axis) of mesenchymal and epithelial cells of healthy and tumor tissue, n = 4; abbreviations: healthy h, tumor t, EpCAM-positive breast cancer cells BCC; (b) To HPRT relative LPAR mRNA expression of different breast (cancer) cell lines, n = 1; (c) Mean maximal ratio of the emission of Fura-2 (340 nm/380 nm excitation) of different breast (cancer) cell lines as effect of 1 μM LPA on cytosolic free calcium levels with the addition of the LPAR antagonist Ki16425, n = 3; (d) The ratio of transmigrated cells compared to the control (y-axis) in varying LPA concentrations (x-axis) of different breast (cancer) cell lines (n = 4) with or without the addition of 2 μM Ki16425; plotted are the means and their SD; *p < 0.0167, Kruskal–Wallis H test, Mann–Whitney U test with Bonferroni correction