Fig. 1

Effects of S100A8 and S100A9 proteins on the secretion of selected cytokines from Panc-1 cells. Conditioned media were collected from untreated cells or cells treated for 24 h with S100A8-GST (A8-GST), S100A9-GST (A9-GST), or treated with recombinant proteins which have been pre-incubated for 1 h with neutralising antibodies (A8-GST + αA8, A9-GST + αA9) or GST protein only. Conditioned media were also collected from cancer cells pre-treated for 1 h with anti-RAGE blocking antibody and then stimulated with recombinant proteins (A8-GST + αRAGE, A9-GST + αRAGE). The cytokine levels were measured using the Bio-Plex Pro 27 Plex Human Cytokine kit, and expressed relative to untreated control levels. Experiments were performed in duplicate three times and error bars denote standard error. * = P values < 0.05