Fig. 7

Compounds D14 and C22 decrease tumor growth in vivo. a Subcutaneous xenograft of cell MIA PaCa-2, the tumor volume was evaluated during the 15 days of treatment. b The Weight of the mice was measured throughout the treatment. The mice were treated with the vehicle and compounds D14 and C22 at the two indicated in the scatter plot. NuNu mice were treated with vehicle (10% DMSO, 0.05% carboxy methyl cellulose and 0.02% Tween 80), D14 at 20 mg kg-1 or C22 at 10 mg kg-1 or 20 mg kg- 1 administered by intraperitoneal injection every two days (n = 10 for DMSO, n = 7 for D14 at 10 mg kg-1, n = 5 for C22 at 10 mg kg-1 and n = 10 for C22 at 20 mg kg-1). Changes in tumor volume are given in relation to the initial volume before treatment (the dotted line indicates the initial size of the tumor). c Hematoxylin-eosin and immunohistochemistry of the tumor sections. The 2 μm sections of the tumors were stained with hematoxylin-eosin (H & E) and analyzed by immunohistochemistry with antibodies directed against cytokeratin 19 (CK19) and Ki-67