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Fig. 4 | BMC Cancer

Fig. 4

From: Activation of antioxidant defences of human mammary epithelial cells under leptin depend on neoplastic state

Fig. 4

Characterization of non-enzymatic and enzymatic lipid peroxidation in the presence of leptin. a: Cell production at 10 min for cytosolic ROS production (DHE). b: Evaluation of lipid hydroperoxides in culture medium at 24 h. c: Evaluation of 8-iso-PGF2α in culture medium at 24 h. d: mRNA expression of cyclo-oxygenase 2 (COX-2) in HMEC. e: mRNA expression COX-2 in MCF-7. f: mRNA expression of COX-2 in MDA-MB-231. g: Catalytic activity of COX-2 in cells at 24 h. Values are expressed as mean ± standard deviation (n = 6). Between-groups comparison was performed by one-way ANOVA followed by the Kruskal-Wallis multiple comparison test. The significance level was set at 0.05. Statistical significance between groups is indicated by * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001

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