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Fig. 5 | BMC Cancer

Fig. 5

From: MEF2 plays a significant role in the tumor inhibitory mechanism of encapsulated RENCA cells via EGF receptor signaling in target tumor cells

Fig. 5

EGFR blockade results in partial attenuation of RENCA macrobead-mediated growth inhibition. (a) In-cell Western analysis of total EGFR, phosphorylated EGFR (pY1068) and overlay (normalized pY1068/EGFR) in DU145/GR cells pre-treated with 1 μM gefitinib (GF) or 0.01% DMSO followed by culture in naïve or 5-day conditioned media from > 18 wk. RENCA macrobeads. Each column represents the mean normalized intensity in arbitrary units (AU) (n = 6–8) ± SD. *p < 0.001, compared with vehicle control or conditioned media. (b) DU145/GR cells pre-treated with 1 μM gefitinib or 0.01% DMSO were cultured with naïve media or together with > 18 wk. RENCA macrobeads in a cell culture insert system for 5 days. Histogram represents percent survival calculated as the percent absorbance of co-cultured samples relative to naïve media samples. Each column represents the mean survival (n = 3) ± SD

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BMC Cancer

ISSN: 1471-2407

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