Skip to main content

Advertisement

Table 1 List of the used antibodies, antigen retrieval (AR), dilutions and Opal™ dyes

From: Expression of the immune checkpoint receptor TIGIT in Hodgkin’s lymphoma

  Bright field Fluorescence
Antibody Target AR Dilution AR Dilution Order1 Dye
DAKO #IR503 CD3 pH 9 1:1 pH 9 1:1 1st Opal 520
DAKO #IR649 CD4 pH 9 1:1 pH 9 1:1 1st Opal 520
DAKO #IR623 CD8 pH 9 1:1 pH 9 1:1 1sta Opal 520b
BioLegend #320102 FOXP3 pH 9 1:50 pH 9 1:50 1st Opal 520
DAKO #IR604 CD20 pH 9 1:1 pH 9 1:10 1sta Opal 520b
Dianova #DIA-TG1 TIGIT pH 7.8 1:70 pH 9 1:150 2nd Opal 570
Abcam #ab52587 PD-1 pH 6 1:50 pH 6 1:50 3rd Opal 690
  1. Order1, order refers to the sequence of antibodies in multiplex fluorescence immunohistochemistry experiments
  2. aantibody was used at third position when stained in combination with CD3, CD4
  3. bwith Opal™ 690 dye