Fig. 6
From: Expression of FGD4 positively correlates with the aggressive phenotype of prostate cancer
Overexpression of FGD4 induced phosphorylation of PAK and cofilin, and filopodia formation. a: Western blot analysis of phospho-PAK and phospho-cofilin in total extracts of C4-2B cells expressing FGD4 or an empty vector showing increased intensity of the peptide bands in FGD4 expressing cells. b: Densitometric analysis of phospho-PAK and phospho-cofilin expression using α-tubulin and GAPDH as the loading controls. Data show mean ± SD of two to three independent experiments. *p<0.0001. c: Immunofluorescence analysis showing filopodia formation (white arrows), accumulation of F-actin and its colocalization with FGD4 (white arrows) at the cell periphery in FGD4-EGFP expressing cells (panels 1, 3 and 5 from the top) compared to control cells, which was not altered upon treatment with ROCK inhibitor (ROCKi; compare panel 3 and panel 5 from the top). Nuclei were stained with DAPI. Scale bar: 10μm.