Fig. 5
From: Expression of FGD4 positively correlates with the aggressive phenotype of prostate cancer
Overexpression of FGD4 promoted migration of prostate cancer cells and altered expression of markers of EMT. a: Quantitative real time PCR analysis of FGD4 mRNA in C4-2B cells transfected with FGD4 pcDNA or the control vector. Expression of RPL13A and GAPDH mRNA were used to normalize FGD4 expression in transfected cells. Data show >3.0- fold increase in FGD4 expression in FGD4 pcDNA transfected C4-2B cells compared to the control cells. b: Migration of C4-2B cells imaged at 24h after transfection with FGD4 expression vector or the empty vector. Four scratches were made in each dish and the experiment was conducted in triplicate. c: Relative rates of migration at 24h. Data represent mean ± SD of three different experiments in quadruplicate wounds. *p<0.03. d: Western blot analysis showing increased expression of expression of SLUG and decreased expression of Ecadherin in FGD4 transfected cells. e. Densitometric analysis of the expression of SLUG and E-cadherin using α-tubulin and GAPDH as the loading controls. Data show mean ± SD of two to three independent experiments. *p=0.005.