Fig. 4
From: Expression of FGD4 positively correlates with the aggressive phenotype of prostate cancer
Inhibition of FGD4 expression reduced migration and activation of CDC42 in prostate cancer cells. a: Migration of PC-3 cells transfected with FGD4 siRNAs or control siRNAs. Migratory properties of PC-3 cells were tested through scratch assays at 0h and 14h after making the scratches. The width of each wound was measured at three areas using light microscopy. Three scratches were made in each dish and the experiment was conducted in triplicate. b: Relative rates of migration at 14h. Data presented as the ratio of the distance traversed by the PC-3 cells transfected with control siRNAs and FGD4 siRNAs. Data represent mean ± SD of three different experiments in triplicate scratches. *p<0.0001. c: Relative concentration of GTP-bound CDC42 in PC-3 cells transfected with FGD4 siRNA or control siRNAs. Data represent mead ± SD of three different experiments. *p<0.01